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Recombinant Human CD40LG Protein (HE0005CL)

Recombinant Human CD40LG Protein (P29965) (149 amino acids) with no tag was expressed in E.coli.
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Price:$290.00
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PRODUCT INFORMATION
Cat.No.
HE0005CL
Synonyms
CD40LG; CD40 ligand; CD40L; CD154; gp39; hCD40L; IGM; IMD3; HIGM1; T-BAM; TNFSF5;
Species
Human
Accession
Source
E. coli
Form
Lyophilized from sterile PBS, pH 7.0.
Bio-activity
The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
Molecular Mass
Recombinant Human CD40LG Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 16.3 kDa.
AA Sequence
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!

BACKGROUND
Background
Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity). Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

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