Recombinant Human CD47 Protein(His tag) (HH0024CL)

Cat.No.

HH0024CL

Synonyms

CD47; IAP; OA3; MER6;

Species

Human

Accession

CD47

Source

HEK293

Tag

His

Predicted N Terminal

Gln 19

Form

Lyophilized from sterile PBS, pH7.4, 10% Trehalose.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD47, His Tag at 2 μg/mL can bind Human SIRP alpha, Fc Tag with a linear range of 0.8-50 ng/mL.

Molecular Mass

Recombinant Human CD47 Protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 14.5 kDa. The reducing protein migrates as 27-40 kDa in SDS-PAGE due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.