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Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Bio-activity
Immobilized Human CTLA-4, Fc Tag at 10 μg/mL (100 ul/well), can bind Human B7-1, His Tag with a linear range of 0.04-4 μg/mL. Immobilized Human B7-2, His Tag at 10 μg/mL (100 ul/well), can bind Human CTLA-4, Fc Tag with a linear range of 0.04-0.3 μg/mL.
Molecular Mass
Recombinant Human CTLA4 Protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. As a result of glycosylation, the reducing protein migrates as 50-55 kDa, and the non-reducing protein migrates as 100-110 kDa in SDS-PAGE.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!
Background
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Tag
Fc
Species
Human
Source
HEK293
BACKGROUND
Background
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
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