Recombinant Human DDR2 Protein(Fc tag) (HH0103DL)

Cat.No.

HH0103DL

Synonyms

DDR2; TKT; MIG20a; NTRKR3; TYRO10;

Species

Human

Accession

DDR2

Source

HEK293

Tag

Fc

Predicted N Terminal

Lys 22

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized Rat tail Collagen I at 10 μg/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2.5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DD

Molecular Mass

Recombinant Human DDR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 616 amino acids and has a calculated molecular mass of 69.4 kDa. Due to glycosylation, rhDDR2/Fc monomer migRates as an approximately 87 kDa protein in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.