Form
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is> 2,500 pmoles/min/μg. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2
Molecular Mass
Recombinant Human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.