Recombinant Human ERBB3 Protein(His tag) (HH0101EL)

Cat.No.

HH0101EL

Synonyms

ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3;

Species

Human

Accession

ERBB3

Source

HEK293

Tag

His

Predicted N Terminal

Ser 20

Form

Lyophilized from sterile PBS, pH 7.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL.

Molecular Mass

Recombinant Human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, Recombinant ErbB3 migRates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
Isoform 2 has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.