Recombinant Human FCGRT&B2M Protein(His tag) (HH0277FL)

Recombinant Human FCGRT Protein (P55899) extracellular domain (Met 1-Ser 297) with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M Protein (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the Human FCGRT&B2M heterodimer was purified.

Datasheet

Size	Price	Qty
20ug	$429.00

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PRODUCT INFORMATION

Cat.No.

HH0277FL

Synonyms

FCGRT; FCRN; B2M

Species

Human

Accession

FCGRT & B2M

Source

HEK293

Tag

His

Predicted N Terminal

Ala 24 & Ile 21

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to bind human IgG1 in a functional ELISA.

Molecular Mass

Recombinant heterodimer of Human FCGRT/B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of rh FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

BACKGROUND

Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

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