Recombinant Human FCGRT&B2M Protein(His tag)

Recombinant Human FCGRT&B2M Protein(His tag) (HH0277FL)

Recombinant Human FCGRT Protein (P55899) extracellular domain (Met 1-Ser 297) with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M Protein (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the Human FCGRT&B2M heterodimer was purified.

Size Price Qty
20ug $390.00

PRODUCT INFORMATION

Cat.No.
HH0277FL
Synonyms
FCGRT; FCRN; B2M
Species
Human
Accession
Source
HEK293
Tag
His
Predicted N Terminal
Ala 24 & Ile 21
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind human IgG1 in a functional ELISA.
Molecular Mass
Recombinant heterodimer of Human FCGRT/B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of rh FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

INQUIRY

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