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Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity
Measured by its binding ability in a functional ELISA. 1 μg/mL of rhFGF-BP was mixed with serially diluted rhFGF basic. Following incubation, the FGFBP-FGF basic complex was captured on a Gt x hFGFBP-coated plate. Bound FGF basic was measured using biotinylated Gt x hFGF basic. The concentration of rhFGF basic that produces 50% of the optimal binding was found to be approximately 5-20 ng/mL.
<1.0 EU per 1 μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Warning
For research use only!
Tag
His
Species
Human
Source
NS0
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