Recombinant Human HMGB1 Protein(His tag) (HH0111HL)

Recombinant Human HMGB1 Protein (P09429) (Met 1-Glu 215) with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus was expressed in HEK293.

Datasheet

Size	Price	Qty
50ug	$396.00

Order Online Bulk Inquiry

PRODUCT INFORMATION

Cat.No.

HH0111HL

Synonyms

HMGB1; HMG3; SBP 1; HMG-1; HMG1; SBP-1;

Species

Human

Accession

HMGB1

Source

HEK293

Tag

His

Predicted N Terminal

Met 1

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml.

Molecular Mass

Recombinant Human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 93 % by SDS-PAGE.

BACKGROUND

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Online Inquiry

Fill out this form and one of our experts will respond to you within one business day.