Form
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Bio-activity
The biological activity of Human IL-10 Rα was determined by its ability to inhibit IL-10 dependent proliferation of MC/9-2 mouse mast cells. The expected ED50=0.05-0.25 µg/ml in the presence of 2 ng/ml or rHuman IL-10.
Each investigator should determine their own optimal working dilution for specific applications.