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Recombinant Human IL13 Protein (HC0116IL)

Recombinant Human IL13 Protein (P35225) (Met 1-Asn 132) with no tag was expressed in CHO.
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Size:20ug
Price:$290.00
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PRODUCT INFORMATION
Cat.No.
HC0116IL
Synonyms
IL13; interleukin 13; interleukin-13; ALRH; BHR1; IL 13; IL-13;
Species
Human
Accession
Source
CHO
Predicted N Terminal
Gly 21
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.). The ED50 for this effect is typically 0.5-3 ng/mL.
Molecular Mass
Recombinant Human IL13 consists of 112 amino acids and migRates as an approximately 12.5 kDa band in SDS-PAGE under reducing conditions as predicted.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 92 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND
Background
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

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