Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized human IL1-beta at 20 μg/ml (100 μl/well) can bind human IL1R1 Fc chimera with a linear ranger of 6.4-160 ng/ml. Immobilized human IL1B-his at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc
Molecular Mass
Recombinant Human IL1R1/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 553 amino acids after removal of the signal peptide and has a predicted molecular mass of 63 kDa. In SDS-PAGE under reducing conditions, it migRates with an apparent molecular mass of 80-90 kDa due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 90 % by SDS-PAGE.
BACKGROUND
Background
Catalyzes the rearrangement of -S-S- bonds in proteins.
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