Recombinant Human IL2RA Protein(His tag) (HH0098IL)

Cat.No.

HH0098IL

Synonyms

IL2RA; IL2R; CD25; p55; IL2-RA; IL-2-RA; TCGFR;

Species

Human

Accession

IL2RA

Source

HEK293

Tag

His

Predicted N Terminal

Glu 22

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant hu

Molecular Mass

Recombinant Human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 97 % by SDS-PAGE.

Background

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.