Form
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL. Measured by its ability to inhibit IL15-dependent proliferation
Molecular Mass
Recombinant Human IL2 Rβ consists of 219 amino acids and has a predicted molecular mass of 25.2 kDa. As a result of glycosylation, the rh IL2 Rβ migRates as an approximately 40 kDa protein in SDS-PAGE under reducing conditions.