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Recombinant Human IL4 Protein (HE0002IL)

Recombinant Human IL4 protein (P05112) (129 amino acids) with no tag was expressed in E.coli.
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Size:20mg
Price:$490.00
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PRODUCT INFORMATION
Cat.No.
HE0002IL
Synonyms
IL4; interleukin-4; BCGF 1; BCGF1; BSF1; IL 4; IL-4; BSF-1; BCGF-1;
Species
Human
Accession
Source
E. coli
Form
Lyophilized from sterile PBS, pH 7.4.
Bio-activity
The ED50 of rHuIL4 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of> 5.0 × 10^6 IU/mg.
Molecular Mass
Recombinant Human IL4 Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 15.0 kDa.
AA Sequence
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!

BACKGROUND
Background
Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity).

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