LIF; CDF; DIA; HILDA; MLPLI;
Lyophilized from sterile PBS, pH 7.4.
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of> 1.0 × 10^7 IU/mg.
Recombinant Human LIF Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 19.7 kDa.
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
< 1.0 EU per 1 microgram of protein (determined by LAL method).
> 98 % by SDS-PAGE.