Recombinant Human LIF Protein(His tag)

Recombinant Human LIF Protein(His tag) (HH0218LL)

Recombinant Human LIF Protein (P15018) (Met1-Phe202) with a C-terminal polyhistidine tag was expressed in HEK293.

Size Price Qty
20ug $290.00

PRODUCT INFORMATION

Cat.No.
HH0218LL
Synonyms
LIF; CDF; DIA; HILDA; MLPLI
Species
Human
Accession
LIF
Source
HEK293
Tag
His
Predicted N Terminal
Ser 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-1 ng/ml.
Molecular Mass
Recombinant Human LIF comprises 191 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is approximately 35-42 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

INQUIRY

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