MEPE; matrix extracellular phosphoglycoprotein; matrix, extracellular phosphoglycoprotein with ASARM motif (bone); osteoblast/osteocyte factor 45; OF45;
Predicted N Terminal
Pro18, Phe20 & Gln21
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rhMEPE, immobilized at 2 µg/mL (100 µL/well), can induce more than 60% of ATDC5 cell adhesion.
Predicted Molecular Mass: 57 kDa
SDS-PAGE: 82 kDa, reducing conditions
Pro18-Asp525 & Phe20-Asp525 & Gln21-Asp525
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain