Form
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is>1000 pmoles/min/μg.
Molecular Mass
Recombinant Human MME/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 936 amino acids and predicts a molecular mass of 106 kDa. As a result of glycosylation, the rh Fc monomer migRates as approximately 120-130 kDa band in SDS-PAGE under reducing conditions.