Recombinant Human NGF Protein

Recombinant Human NGF Protein (HC0166NL)

Recombinant Human NGF Protein (P01138) (Ser 122-Arg 239) with no tag was expressed in CHO.

Size Price Qty
100ug $539.00

PRODUCT INFORMATION

Cat.No.
HC0166NL
Synonyms
NGF; NGFB; HSAN5; Beta-NGF
Species
Human
Accession
NGF
Source
CHO
Predicted N Terminal
Ser 122
Form
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
Molecular Mass
Recombinant Human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).

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