Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to hydrolyze the 5'phosphate group from the substrate adenosine 5'monophosphate (AMP). The specific activity is> 20,000 pmoles/min/μg.
Molecular Mass
Recombinant Human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 85 % by SDS-PAGE.
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