Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately>60% will adhere specifically after 30 minutes at 37?C.
Molecular Mass
Recombinant Human POSTN consists of 771 amino acids and has a predicted molecular mass of 86 kDa. In SDS-PAGE under reducing conditions, it migRates with an apparent molecular mass of 80 kDa due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.
BACKGROUND
Background
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Online Inquiry
Fill out this form and one of our experts will respond to you within one business day.