Recombinant Human SIRPA Protein(Fc tag) (HC0058SL)

Cat.No.

HC0058SL

Synonyms

SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; MYD-1; CD172A; PTPNS1;

Species

Human

Accession

SIRPA

Source

CHO

Tag

Fc

Predicted N Terminal

Glu 31

Form

Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD47, His Tag at 2 μg/mL can bind Human SIRP alpha, Fc Tag (HPLC-verified) with a linear range of 0.8-50 ng/mL.

Molecular Mass

Recombinant Human SIRPA Protein is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The reducing protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.