Recombinant Human SIRPA Protein(His tag) (HH0187SL)

Cat.No.

HH0187SL

Synonyms

SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRPalpha; SIRPalpha2; MYD-1; CD172A; PTPNS1

Species

Human

Accession

SIRPA

Source

HEK293

Tag

His

Predicted N Terminal

Glu 31

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.

Molecular Mass

Recombinant Human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPA is approximately 45-50 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 98 % by SDS-PAGE.

Background

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.