Lyophilized from sterile 20mM NaAC, 200mM NaCl, pH 5.5, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 6 nM.
Molecular Mass
Recombinant Human TIMP1 comprises 184 amino acids with a predicted molecular mass of 21 kDa. It migRates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.
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