Recombinant Human TNF Protein(Flag tag) (HH1157TS)

Recombinant Human TNF protein with an N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293.
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PRODUCT INFORMATION

Cat.No.
HH1157TS
Synonyms
TNF; TNFA; TNF-alpha;
Species
Human
Accession
Source
HEK293
Tag
Flag
Form
Lyophilized. Contains PBS.
Bio-activity
Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Molecular Mass
~34kDa (SDS-PAGE)
Protein length
aa 85-233
Endotoxin
<0.02EU/μg purified protein (LAL test; Lonza).
Purity
≥95% (SDS-PAGE)

Usage Guide

Stability
Stable for at least 6 months after receipt when stored at -20 centigrade.
Storage
Short Term Storage: +4 centigrade. Long Term Storage: -20 centigrade. Handling Advice After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration
0.1mg/ml after reconstitution.
Reconstitution
Reconstitute with 100μl sterile water.
Warning
For research use only!


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