Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 5 % trehalose and 5 % mannitol.
Molecular Mass
Recombinant Human USP5 consists of 846 amino acids and predicts a molecular mass of 94.7 kDa. It migRates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 92 % by SDS-PAGE.
BACKGROUND
Background
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
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