Recombinant Human PTH Protein (HE0010PS)

Cat.No.

HE0010PS

Synonyms

PTH; parathyroid hormone; parathormone; parathyrin; PTH1

Accession

PTH

Form

Liquid. In phosphate-buffered Saline(pH 7.4) containing 2 mM EDTA

Bio-activity

Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.

Molecular Mass

9.55 kDa (85 aa), confirmed by MALDI-TOF

AA Sequence

MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/ml (determined by Bradford assay)

Warning

For research use only!

Background

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants.

References

Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88(11), 5212-20.
Wingender E., et al. (1989) J. Biol. Chem. 264(8),4367-73.

Species

Human

Source

E. coli

Background

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants.

References

Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88(11), 5212-20.
Wingender E., et al. (1989) J. Biol. Chem. 264(8),4367-73.