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Recombinant Mouse Cd274 Protein(His tag) (MH0221CL)

Recombinant Mouse Cd274 Protein (Q9EP73) (Met 1-Thr 238) with a polyhistidine tag at the C-terminus was expressed in HEK293.
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Size:100ug
Price:$390.00
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PRODUCT INFORMATION
Cat.No.
MH0221CL
Synonyms
CD274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1
Species
Mouse
Accession
Source
HEK293
Tag
His
Predicted N Terminal
Phe 19
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD1-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml.
Molecular Mass
Recombinant Mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migRates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND
Background
Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

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