Recombinant Mouse Egfr Protein(His tag) (MH0043EL)

Cat.No.

MH0043EL

Synonyms

EGFR; waved 2; Wa5; wa2; Erbb; Errp; wa-2;

Species

Mouse

Accession

Egfr

Source

HEK293

Tag

His

Predicted N Terminal

Leu 25

Form

Lyophilized from sterile PBS, pH7.4, 10% Trehalose.

Molecular Mass

Recombinant Mouse Egfr Protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 70.1 kDa. The reducing protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.