Recombinant Mouse Fgfr1 Protein(His tag)

Recombinant Mouse Fgfr1 Protein(His tag) (MH0233FL)

Recombinant Mouse Fgfr1 Protein (P16092) (Met 1-Glu 376) with a polyhistidine tag at the C-terminus was expressed in HEK293.

Size Price Qty
50ug $390.00

PRODUCT INFORMATION

Cat.No.
MH0233FL
Synonyms
FGFR1; MFR; c-fgr; bFGF-R-1; FLG; Eask; Hspy; Flt-2; Fgfr-1
Species
Mouse
Accession
Source
HEK293
Tag
His
Predicted N Terminal
Arg 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 50-200 ng/ml.
Molecular Mass
Recombinant Mouse FGFR1 consists of 366 amino acids and has a calculated molecular mass of 40.9 kDa. The apparent molecular mass of rmFGFR1 is approximately 55-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation (By similarity).

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