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Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind biotinylated human FGF basic in a functional ELISA.
Molecular Mass
Recombinant Mouse GPC3 comprises 544 amino acids and has a predicted molecular mass of 62 kDa. The apparent molecular mass of the protein is approximately 62 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition (By similarity).
Tag
His
Species
Mouse
Source
Insect cells
BACKGROUND
Background
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition (By similarity).
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