Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind biotinylated human FGF basic in a functional ELISA.
Molecular Mass
Recombinant Mouse GPC3 comprises 544 amino acids and has a predicted molecular mass of 62 kDa. The apparent molecular mass of the protein is approximately 62 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
BACKGROUND
Background
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition (By similarity).
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