Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.0, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant mouse Hemopexin binds>15 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of mouse Hemopexin.
Molecular Mass
Recombinant Mouse HPX consists of 448 amino acids and has a predicted molecular mass of 50.4 kDa. The apparent molecular mass of the rm HPX is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.
BACKGROUND
Background
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Online Inquiry
Fill out this form and one of our experts will respond to you within one business day.