IL3; interleukin 3; interleukin-3; mast cell growth factor; Il-3; MCGF; Csfmu;
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.
135 amino acids
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IL-3 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
For research use only!