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Recombinant Mouse Il4 Protein (ME0013IL)

Recombinant Mouse Il4 Protein (P07750) (121 amino acids) with no tag was expressed in E.coli.
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Size:20ug
Price:$290.00
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PRODUCT INFORMATION
Cat.No.
ME0013IL
Synonyms
IL4; interleukin-4; Il-4; BSF-1;
Species
Mouse
Accession
Source
E. coli
Form
Lyophilized from sterile PBS, pH 7.4.
Bio-activity
The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of> 5 × 10^5 IU/mg.
Molecular Mass
Recombinant Mouse Il4 Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 13.5 kDa.
AA Sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!

BACKGROUND
Background
Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3083412). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells (PubMed:3498301). It enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:3498301). It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (PubMed:25847241).

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