Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is>10,000 pmol/min/?g.
Molecular Mass
Recombinant Mouse NT5E consists of 532 amino acids and has a calculated molecular mass of 59.4 kDa which is also detected in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
BACKGROUND
Background
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Online Inquiry
Fill out this form and one of our experts will respond to you within one business day.