Recombinant Mouse Pigr Protein(His tag)

Recombinant Mouse Pigr Protein(His tag) (MH0229PL)

Recombinant Mouse Pigr Protein (O70570) (Met 1-Lys 645) with a C-terminal polyhistidine tag was expressed in HEK293.

Size Price Qty
50ug $390.00

PRODUCT INFORMATION

Cat.No.
MH0229PL
Synonyms
PIGR; poly-Ig receptor
Species
Mouse
Accession
Source
HEK293
Tag
His
Predicted N Terminal
Lys 19
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PIGR at 5 μg/ml (100 μl/well) can bind mouse IgM with a linear range of 0.156-10 μg/ml.
Molecular Mass
Recombinant Mouse PIGR consists of 638 amino acids and has a calculated molecular mass of 70.8 kDa. As a result of glycosylation, Recombinant protein migRates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment (By similarity).

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