Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Molecular Mass
Recombinant Mouse PLA2G1B consists of 142 amino acids and has a predicted molecular mass of 16.3 kDa. rmPLA2G1B migRates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 96 % by SDS-PAGE.
BACKGROUND
Background
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
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