Recombinant Mouse Sirpa Protein(Fc tag) (MH0258SL)

Cat.No.

MH0258SL

Synonyms

SIRPA; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1

Species

Mouse

Accession

Sirpa

Source

HEK293

Tag

Fc

Predicted N Terminal

Gly 26

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Immobilized mouse PVR-His at 10 μg/ml (100 μl/well) can bind mouse TIGIT-Fc, The EC50 of mouse TIGIT-Fc is 0.25-0.55 μg/ml.

Molecular Mass

Recombinant Mouse SIRPA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a calculated molecular mass of 64.9 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA/Fc monomer is approximately 1001-20 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Background

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).