Recombinant Mouse Timp1 Protein(His tag) (MH0243TL)

Recombinant Mouse Timp1 Protein (P12032) (Met 1-Arg 205) with a C-terminal polyhistidine tag was expressed in HEK293.

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PRODUCT INFORMATION

Cat.No.

MH0243TL

Synonyms

TIMP1; EPA; TPA-S1; Clgi; Timp; TIMP-1

Species

Mouse

Accession

Timp1

Source

HEK293

Tag

His

Predicted N Terminal

Cys 25

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 6 nM.

Molecular Mass

Recombinant Mouse TIMP1 consists of 181 amino acids and has a calculated molecular mass of 21 kDa. It migRates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

BACKGROUND

Background

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

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