Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse 4-1BB Ligand at 20 μg/ml (100ul/well) can bind human 4-1BB with a linear range of 15.6-500 ng/ml.
Molecular Mass
Recombinant Mouse TNFSF9 consists of 222 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, Recombinant protein migRates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.
BACKGROUND
Background
Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
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