Recombinant Mouse Ctsl Protein (MI0011CS)

Cat.No.

MI0011CS

Synonyms

Cathepsin L1; CTSL; CatL; Ctsl1; fs; MEP; nkt

Accession

Ctsl

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Molecular Mass

36.8kDa (325aa)
40-57KDa (SDS-PAGE under reducing conditions.)

AA Sequence

TPKFDQTFSA EWHQWKSTHR RLYGTNEEEW RRAIWEKNMR MIQLHNGEYS NGQHGFSMEM NAFGDMTNEE FRQVVNGYRH QKHKKGRLFQEPLMLKIPKS VDWREKGCVT PVKNQGQCGS CWAFSASGCL EGQMFLKTGK LISLSEQNLV DCSHAQGNQG CNGGLMDFAF QYIKENGGLDSEESYPYEAK DGSCKYRAEF AVANDTGFVD IPQQEKALMK AVATVGPISV AMDASHPSLQ FYSSGIYYEP NCSSKNLDHG VLLVGYGYEGTDSNKNKYWL VKNSWGSEWG MEGYIKIAKD RDNHCGLATA ASYPVVNLEH HHHHH

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.25mg/ml (determined by Absorbance at 280nm)

Warning

For research use only!

Background

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome.

References

Goretzki L., et al. (1992) FEBS Lett. 297:112-118.
Taggart CC., et al. (2001) J Biol Chem. 276:33345-33352.

Tag

His tag

Species

Mouse

Source

Insect cells

Background

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome.

References

Goretzki L., et al. (1992) FEBS Lett. 297:112-118.
Taggart CC., et al. (2001) J Biol Chem. 276:33345-33352.