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Recombinant Rat Fcgr2b Protein(His tag) (RH0263FL)

Recombinant Rat Fcgr2b Protein (Q63203) (Met 1-Pro 212) with a polyhistidine tag at the C-terminus was expressed in HEK293.
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Price:$390.00
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PRODUCT INFORMATION
Cat.No.
RH0263FL
Synonyms
FCGR2B; FC-gamma RII; FCRII; Fcgr2
Species
Rat
Accession
Source
HEK293
Tag
His
Predicted N Terminal
His 32
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized human rat FCGR2B-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.3-0.8 μg/ml.
Molecular Mass
Recombinant Rat FCGR2B comprises 192 amino acids and predicts a molecular mass of 21.9 kDa. The apparent molecular mass of the Rat FCGR2B is approximately 35-40 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND
Background
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.

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