Soil Aryl-acylamidase Activity Assay Kit (SESAS-K066M)

Cat.No.

SESAS-K066M

Method

Microplate-based method

Product size

48T

Note

Provide microplate

Product description

Soil aryl-acylamidase (Aryl-acylamidase, EC 3.5.1.13) can hydrolyze compounds with amide groups; this kit uses soil aryl-acylamidase to hydrolyze the substrate L-leucine β-naphthylamide to generate β-naphthylamine, which then reacts with p-dimethylaminocinnamaldehyde to form a red azo compound with a characteristic absorption peak at 540nm. By detecting the increase rate of this red substance, the activity of soil aryl-acylamidase can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, balance, centrifuge, temperature-controlled water bath, ethanol, pipettes.