Soil β-Galactosidase (S-β-GAL) Assay Kit (SESAS-K077S)

Cat.No.

SESAS-K077S

Method

Spectrophotometry

Product size

24T

Product description

Soil β-galactosidase (β-GAL, EC 3.2.1.23), also known as β-D-galactoside galactosyltransferase, is involved in the hydrolysis of carbohydrates in soil. This kit provides a simple, sensitive, and rapid detection method. β-GAL decomposes p-nitrophenyl-β-D-galactopyranoside to produce p-nitrophenol (PNP), which has the maximum absorption peak at 405nm. By measuring the rate of increase in absorbance, the activity of β-GAL can be calculated.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, temperature-controlled shaking incubator/water bath, pipettes, balance.