Product description
Soil phosphatase plays an important role in the availability of soil phosphorus and is an indicator for evaluating the direction and intensity of soil phosphorus biotransformation. It also has a certain relationship with soil carbon, nitrogen content, available phosphorus content, and pH. This kit provides a simple, sensitive, and rapid detection method. In an alkaline environment, soil alkaline phosphatase (S-LP) catalyzes the generation of yellow p-nitrophenol (PNP) from p-nitrophenyl phosphate (PNPP), which has a maximum absorption peak at 405nm. By detecting the increase rate of PNP at 405nm, the activity of S-ALP enzyme can be obtained.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, incubator, analytical balance, pipettes, distilled water, toluene.