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Sorbitol is widely found in animals, plants, and microorganisms, serving as a form of sugar transport and is related to biological stress resistance. It can be used as a food additive to enhance food flavor. In studies of sugar metabolism, stress resistance, and food research, it is often necessary to detect the content of sorbitol. Sorbitol forms a blue complex with copper ions in an alkaline solution, which has a characteristic absorption peak at 655nm, thereby determining the content of sorbitol in the sample.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.