Product description
Superoxide anion radicals, as one of the free radicals produced in the metabolic process of organisms, can attack macromolecules, causing damage to cell structure and function, and are closely related to aging and diseases of the body. The study of scavenging superoxide anion radicals has received widespread attention. Under weakly alkaline conditions, phenol can undergo auto-oxidation to produce superoxide anions and colored intermediate products, which have a characteristic absorption peak at 320nm. When a superoxide anion scavenger is added, it can quickly react with superoxide anions to prevent the accumulation of intermediate products, thereby reducing the light absorption at 320nm. The scavenging ability of the scavenger against superoxide anions can be evaluated by measuring the A320 value.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, balance, centrifuge.