Product description
Total bilirubin (TBIL) is oxidized by sodium nitrite in the presence of the surfactant Triton-X100 to form biliverdin, and the decrease in absorbance at 450nm is proportional to the concentration of total bilirubin, allowing for the determination of TBIL content.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, centrifuge, distilled water.